Chen-Ming Zhang, Susan A. Reslewic, Charles E. Glatz Abstract This work demonstrates that proper selection of a metal ion and chelating ligand enables recovery of a his6-tagged protein from canola (Brassica napus) extracts by immobilized metal affinity chromatography (IMAC). When using Co2+ with iminodiacetate (IDA) as the chelating ligand, β-glucuronidase-his6 (GUSH6) can be purified from canola protein extract with almost […]
Suitability of immobilized metal affinity chromatography for protein purification from canola
